bluescript ii sk1 vector Search Results


99
New England Biolabs pbluescript sk 1 vector
Pbluescript Sk 1 Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbluescript sk 1 vector/product/New England Biolabs
Average 99 stars, based on 1 article reviews
pbluescript sk 1 vector - by Bioz Stars, 2026-06
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90
Becton Dickinson mouse igg mabs detecting human cd8
Enumeration and in situ staining of immune cells in ectocervical tissue. (A) Distribution and median of the percentage of total cells in the ectocervical epithelium and submucosa of tissue samples from the three study groups. A nonparametric, two-tailed Mann–Whitney U test was used to compare the HIV+FSW group versus the HIV−FSW group and the HIV+FSW group versus the HIV−LR group. (B) Immunofluorescent images of ectocervical tissue sections from an HIV+FSW subject. The image on the right for each pair is a magnified view of the region indicated in the box in the image to the left. The majority of <t>CD8+</t> cells (red) were also CD3+ (green) and vice versa (left panels); the majority of CD3+ cells (red) were not CD4+ cells (green) (right panels). Scale bar, 100 μm. (C) Bright-field images of ectocervical tissue stained with hematoxylin (blue) for visualization of cell nuclei and stained for CD8+ cells (brown; upper panels) and CD3+ cells (brown; lower panels). Scale bar, 200 μm.
Mouse Igg Mabs Detecting Human Cd8, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse igg mabs detecting human cd8/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
mouse igg mabs detecting human cd8 - by Bioz Stars, 2026-06
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90
Promega pbluescript sk(1) vectors
Enumeration and in situ staining of immune cells in ectocervical tissue. (A) Distribution and median of the percentage of total cells in the ectocervical epithelium and submucosa of tissue samples from the three study groups. A nonparametric, two-tailed Mann–Whitney U test was used to compare the HIV+FSW group versus the HIV−FSW group and the HIV+FSW group versus the HIV−LR group. (B) Immunofluorescent images of ectocervical tissue sections from an HIV+FSW subject. The image on the right for each pair is a magnified view of the region indicated in the box in the image to the left. The majority of <t>CD8+</t> cells (red) were also CD3+ (green) and vice versa (left panels); the majority of CD3+ cells (red) were not CD4+ cells (green) (right panels). Scale bar, 100 μm. (C) Bright-field images of ectocervical tissue stained with hematoxylin (blue) for visualization of cell nuclei and stained for CD8+ cells (brown; upper panels) and CD3+ cells (brown; lower panels). Scale bar, 200 μm.
Pbluescript Sk(1) Vectors, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbluescript sk(1) vectors/product/Promega
Average 90 stars, based on 1 article reviews
pbluescript sk(1) vectors - by Bioz Stars, 2026-06
90/100 stars
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90
Merck KGaA pbluescriptii sk1 vectors
Enumeration and in situ staining of immune cells in ectocervical tissue. (A) Distribution and median of the percentage of total cells in the ectocervical epithelium and submucosa of tissue samples from the three study groups. A nonparametric, two-tailed Mann–Whitney U test was used to compare the HIV+FSW group versus the HIV−FSW group and the HIV+FSW group versus the HIV−LR group. (B) Immunofluorescent images of ectocervical tissue sections from an HIV+FSW subject. The image on the right for each pair is a magnified view of the region indicated in the box in the image to the left. The majority of <t>CD8+</t> cells (red) were also CD3+ (green) and vice versa (left panels); the majority of CD3+ cells (red) were not CD4+ cells (green) (right panels). Scale bar, 100 μm. (C) Bright-field images of ectocervical tissue stained with hematoxylin (blue) for visualization of cell nuclei and stained for CD8+ cells (brown; upper panels) and CD3+ cells (brown; lower panels). Scale bar, 200 μm.
Pbluescriptii Sk1 Vectors, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbluescriptii sk1 vectors/product/Merck KGaA
Average 90 stars, based on 1 article reviews
pbluescriptii sk1 vectors - by Bioz Stars, 2026-06
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90
Promega pbsii sk1 vector
Enumeration and in situ staining of immune cells in ectocervical tissue. (A) Distribution and median of the percentage of total cells in the ectocervical epithelium and submucosa of tissue samples from the three study groups. A nonparametric, two-tailed Mann–Whitney U test was used to compare the HIV+FSW group versus the HIV−FSW group and the HIV+FSW group versus the HIV−LR group. (B) Immunofluorescent images of ectocervical tissue sections from an HIV+FSW subject. The image on the right for each pair is a magnified view of the region indicated in the box in the image to the left. The majority of <t>CD8+</t> cells (red) were also CD3+ (green) and vice versa (left panels); the majority of CD3+ cells (red) were not CD4+ cells (green) (right panels). Scale bar, 100 μm. (C) Bright-field images of ectocervical tissue stained with hematoxylin (blue) for visualization of cell nuclei and stained for CD8+ cells (brown; upper panels) and CD3+ cells (brown; lower panels). Scale bar, 200 μm.
Pbsii Sk1 Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbsii sk1 vector/product/Promega
Average 90 stars, based on 1 article reviews
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90
Promega bluescript sk1 vector
Enumeration and in situ staining of immune cells in ectocervical tissue. (A) Distribution and median of the percentage of total cells in the ectocervical epithelium and submucosa of tissue samples from the three study groups. A nonparametric, two-tailed Mann–Whitney U test was used to compare the HIV+FSW group versus the HIV−FSW group and the HIV+FSW group versus the HIV−LR group. (B) Immunofluorescent images of ectocervical tissue sections from an HIV+FSW subject. The image on the right for each pair is a magnified view of the region indicated in the box in the image to the left. The majority of <t>CD8+</t> cells (red) were also CD3+ (green) and vice versa (left panels); the majority of CD3+ cells (red) were not CD4+ cells (green) (right panels). Scale bar, 100 μm. (C) Bright-field images of ectocervical tissue stained with hematoxylin (blue) for visualization of cell nuclei and stained for CD8+ cells (brown; upper panels) and CD3+ cells (brown; lower panels). Scale bar, 200 μm.
Bluescript Sk1 Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bluescript sk1 vector/product/Promega
Average 90 stars, based on 1 article reviews
bluescript sk1 vector - by Bioz Stars, 2026-06
90/100 stars
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90
Promega pcr-script sk (1) cloning vector
Enumeration and in situ staining of immune cells in ectocervical tissue. (A) Distribution and median of the percentage of total cells in the ectocervical epithelium and submucosa of tissue samples from the three study groups. A nonparametric, two-tailed Mann–Whitney U test was used to compare the HIV+FSW group versus the HIV−FSW group and the HIV+FSW group versus the HIV−LR group. (B) Immunofluorescent images of ectocervical tissue sections from an HIV+FSW subject. The image on the right for each pair is a magnified view of the region indicated in the box in the image to the left. The majority of <t>CD8+</t> cells (red) were also CD3+ (green) and vice versa (left panels); the majority of CD3+ cells (red) were not CD4+ cells (green) (right panels). Scale bar, 100 μm. (C) Bright-field images of ectocervical tissue stained with hematoxylin (blue) for visualization of cell nuclei and stained for CD8+ cells (brown; upper panels) and CD3+ cells (brown; lower panels). Scale bar, 200 μm.
Pcr Script Sk (1) Cloning Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcr-script sk (1) cloning vector/product/Promega
Average 90 stars, based on 1 article reviews
pcr-script sk (1) cloning vector - by Bioz Stars, 2026-06
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Image Search Results


Enumeration and in situ staining of immune cells in ectocervical tissue. (A) Distribution and median of the percentage of total cells in the ectocervical epithelium and submucosa of tissue samples from the three study groups. A nonparametric, two-tailed Mann–Whitney U test was used to compare the HIV+FSW group versus the HIV−FSW group and the HIV+FSW group versus the HIV−LR group. (B) Immunofluorescent images of ectocervical tissue sections from an HIV+FSW subject. The image on the right for each pair is a magnified view of the region indicated in the box in the image to the left. The majority of CD8+ cells (red) were also CD3+ (green) and vice versa (left panels); the majority of CD3+ cells (red) were not CD4+ cells (green) (right panels). Scale bar, 100 μm. (C) Bright-field images of ectocervical tissue stained with hematoxylin (blue) for visualization of cell nuclei and stained for CD8+ cells (brown; upper panels) and CD3+ cells (brown; lower panels). Scale bar, 200 μm.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Presence of CD8 + T Cells in the Ectocervical Mucosa Correlates with Genital Viral Shedding in HIV-Infected Women despite a Low Prevalence of HIV RNA-Expressing Cells in the Tissue

doi: 10.4049/jimmunol.1302826

Figure Lengend Snippet: Enumeration and in situ staining of immune cells in ectocervical tissue. (A) Distribution and median of the percentage of total cells in the ectocervical epithelium and submucosa of tissue samples from the three study groups. A nonparametric, two-tailed Mann–Whitney U test was used to compare the HIV+FSW group versus the HIV−FSW group and the HIV+FSW group versus the HIV−LR group. (B) Immunofluorescent images of ectocervical tissue sections from an HIV+FSW subject. The image on the right for each pair is a magnified view of the region indicated in the box in the image to the left. The majority of CD8+ cells (red) were also CD3+ (green) and vice versa (left panels); the majority of CD3+ cells (red) were not CD4+ cells (green) (right panels). Scale bar, 100 μm. (C) Bright-field images of ectocervical tissue stained with hematoxylin (blue) for visualization of cell nuclei and stained for CD8+ cells (brown; upper panels) and CD3+ cells (brown; lower panels). Scale bar, 200 μm.

Article Snippet: In brief, endogenous biotin was blocked using an Avidin/Biotin Blocking Kit (Vector Laboratories, Burlingame, CA), followed by the addition of selected mouse IgG mAbs detecting human CD3 (clone SK7), CD8 (clone SK1), or HLA-DR (clone L243; all from BD Biosciences, Stockholm, Sweden).

Techniques: In Situ, Staining, Two Tailed Test, MANN-WHITNEY

Enumeration of CD8+ and CD3+ cells in ectocervical tissue. Distribution and median of the percentage of positively stained (A) CD8+ cells in the total tissue analyzed (B) and in the ectocervical epithelial and submucosal tissue area analyzed separately. Percentage of positively stained (C) CD3+ cells in the total tissue analyzed and (D) in the ectocervical epithelial and sub-mucosal tissue area analyzed separately, as assessed by imaging analysis. (E) CD8+/CD3+ cell ratio in the total ectocervical epithelial area and submucosal area. (F) Correlation between the percentage of CD8+ cells and the percentage of the total cells within the ectocervical tissue area analyzed by imaging analysis. (G) Distribution and median relative quantification (RQ; UBC = 1) of CD8 mRNA expression, which was assessed by qPCR. (H) CD8/CD3 mRNA ratio. The Ct values for each target gene were normalized to UBC. Fold change of the target genes was calculated as 2−dCt. A nonparametric, two-tailed Mann–Whitney U test was used to analyze the statistical significance between the study groups.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Presence of CD8 + T Cells in the Ectocervical Mucosa Correlates with Genital Viral Shedding in HIV-Infected Women despite a Low Prevalence of HIV RNA-Expressing Cells in the Tissue

doi: 10.4049/jimmunol.1302826

Figure Lengend Snippet: Enumeration of CD8+ and CD3+ cells in ectocervical tissue. Distribution and median of the percentage of positively stained (A) CD8+ cells in the total tissue analyzed (B) and in the ectocervical epithelial and submucosal tissue area analyzed separately. Percentage of positively stained (C) CD3+ cells in the total tissue analyzed and (D) in the ectocervical epithelial and sub-mucosal tissue area analyzed separately, as assessed by imaging analysis. (E) CD8+/CD3+ cell ratio in the total ectocervical epithelial area and submucosal area. (F) Correlation between the percentage of CD8+ cells and the percentage of the total cells within the ectocervical tissue area analyzed by imaging analysis. (G) Distribution and median relative quantification (RQ; UBC = 1) of CD8 mRNA expression, which was assessed by qPCR. (H) CD8/CD3 mRNA ratio. The Ct values for each target gene were normalized to UBC. Fold change of the target genes was calculated as 2−dCt. A nonparametric, two-tailed Mann–Whitney U test was used to analyze the statistical significance between the study groups.

Article Snippet: In brief, endogenous biotin was blocked using an Avidin/Biotin Blocking Kit (Vector Laboratories, Burlingame, CA), followed by the addition of selected mouse IgG mAbs detecting human CD3 (clone SK7), CD8 (clone SK1), or HLA-DR (clone L243; all from BD Biosciences, Stockholm, Sweden).

Techniques: Staining, Imaging, Expressing, Two Tailed Test, MANN-WHITNEY

Comparisons between cellular markers versus plasma and cervical viral load. Correlation between plasma viral load (left panels) and cervical viral load (right panels) and the percentage of CD8+ cells (A) within the total ectocervical tissue area (B), the epithelial tissue area, and (C) the submucosal tissue area, as assessed by imaging analysis. Relative quantification (RQ; UBC = 1) of (D) CD8 mRNA and (E) CD69 mRNA levels, as assessed by qPCR. Spearman rank correlation coefficient test was used to assess correlations.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Presence of CD8 + T Cells in the Ectocervical Mucosa Correlates with Genital Viral Shedding in HIV-Infected Women despite a Low Prevalence of HIV RNA-Expressing Cells in the Tissue

doi: 10.4049/jimmunol.1302826

Figure Lengend Snippet: Comparisons between cellular markers versus plasma and cervical viral load. Correlation between plasma viral load (left panels) and cervical viral load (right panels) and the percentage of CD8+ cells (A) within the total ectocervical tissue area (B), the epithelial tissue area, and (C) the submucosal tissue area, as assessed by imaging analysis. Relative quantification (RQ; UBC = 1) of (D) CD8 mRNA and (E) CD69 mRNA levels, as assessed by qPCR. Spearman rank correlation coefficient test was used to assess correlations.

Article Snippet: In brief, endogenous biotin was blocked using an Avidin/Biotin Blocking Kit (Vector Laboratories, Burlingame, CA), followed by the addition of selected mouse IgG mAbs detecting human CD3 (clone SK7), CD8 (clone SK1), or HLA-DR (clone L243; all from BD Biosciences, Stockholm, Sweden).

Techniques: Imaging

Enumeration of HLA-DR+ cells and HLA-DR mRNA expression in ectocervical tissue. Distribution and median of the percentage of positively stained HLA-DR+ cells in (A) the total tissue analyzed (B) and in the ectocervical epithelial and submucosal tissue area analyzed separately, as assessed by imaging analysis. (C) Relative quantification (RQ; UBC = 1) of HLA-DR mRNA expression, which was assessed by qPCR. A nonparametric, two-tailed Mann–Whitney U test was used to analyze statistical significance between the HIV+FSW group versus the HIV−FSW group and the HIV+FSW group versus the HIV−LR group. (D) Bright-field images of human ectocervical tissue stained with hematoxylin (blue) for visualization of cell nuclei and stained for HLA-DR (brown). Scale bars, 200 μm. (E) Correlation between the percentage of CD8+ cells and percentage of HLA-DR+ cells within the total ectocervical tissue area, as assessed by imaging analysis in the HIV+FSW group. (F) Correlation between the RQ of CD8 and HLA-DR mRNA levels, as assessed by qPCR, in the HIV+FSW group. Spearman rank correlation coefficient test was used to assess the correlation.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Presence of CD8 + T Cells in the Ectocervical Mucosa Correlates with Genital Viral Shedding in HIV-Infected Women despite a Low Prevalence of HIV RNA-Expressing Cells in the Tissue

doi: 10.4049/jimmunol.1302826

Figure Lengend Snippet: Enumeration of HLA-DR+ cells and HLA-DR mRNA expression in ectocervical tissue. Distribution and median of the percentage of positively stained HLA-DR+ cells in (A) the total tissue analyzed (B) and in the ectocervical epithelial and submucosal tissue area analyzed separately, as assessed by imaging analysis. (C) Relative quantification (RQ; UBC = 1) of HLA-DR mRNA expression, which was assessed by qPCR. A nonparametric, two-tailed Mann–Whitney U test was used to analyze statistical significance between the HIV+FSW group versus the HIV−FSW group and the HIV+FSW group versus the HIV−LR group. (D) Bright-field images of human ectocervical tissue stained with hematoxylin (blue) for visualization of cell nuclei and stained for HLA-DR (brown). Scale bars, 200 μm. (E) Correlation between the percentage of CD8+ cells and percentage of HLA-DR+ cells within the total ectocervical tissue area, as assessed by imaging analysis in the HIV+FSW group. (F) Correlation between the RQ of CD8 and HLA-DR mRNA levels, as assessed by qPCR, in the HIV+FSW group. Spearman rank correlation coefficient test was used to assess the correlation.

Article Snippet: In brief, endogenous biotin was blocked using an Avidin/Biotin Blocking Kit (Vector Laboratories, Burlingame, CA), followed by the addition of selected mouse IgG mAbs detecting human CD3 (clone SK7), CD8 (clone SK1), or HLA-DR (clone L243; all from BD Biosciences, Stockholm, Sweden).

Techniques: Expressing, Staining, Imaging, Two Tailed Test, MANN-WHITNEY